我国华东地区两种茶尺蛾的形态和分子鉴定

摘要我国华东地区两种悲哀为害茶树的益虫灰茶尺蛾Ectropin grisescens Warren,1894和茶作线环的人外倾 obliqua (普罗特,1915)齐式比拟、分级不清,农业生产中常常暧昧不明,它给举行辩护和举行辩护任务的开展产量了相当大的难事。。为了精确的辨别灰茶尺蛾和茶作线环的人,两种益虫Carri的塑造留心和分子剖析。对灰茶尺蛾和茶作线环的人成年的、卵、比拟剖析了根除和蛹的塑造特点。;并对灰茶尺蛾和茶作线环的人的39个范本的细胞形成核COI生殖细胞的细胞质举行了发挥和测序,两个物种经过的种内和种间遗传间隔为,由于Kimura-2参量形成图案,用ML和NJ办法构成体系树。灰茶尺蛾和茶作线环的人嫁妆塑造学特点具有明显离题;分子通知显示,在T细胞中有一明显的条形码。 gap”,功能ML和NJ法构成的体系发育树可以将灰茶尺蛾和茶作线环的人成的区别开。由于COI生殖细胞的细胞质的分子评议树或花草结果与MORP分歧。,可用于益虫防治及互相牵连详细地检查。。外倾 grisescens Warren, 1894 and 外倾 obliqua (普罗特,1915),two serious pests of tea in south-eastern China, are morphologically similar and cannot easily be distinguished, which makes it difficult to conduct research on these 益虫。 To accurately identify E. grisescens and E. obliqua,we examined and compared morphological characters and the mitochondrial CO I gene sequences of these two 种。 [办法] Morphological characters of adults, eggs, larvae and pupae of each species were observed, illustrated, measured and 相形。 39 samples of the mitochondrial CO I gene of E. grisescens and E. obliqua were amplified and sequenced, and the intraspecific and interspecific divergences of the two species were 计算。 NJ and ML trees were built based on the Kimura-2-parameter 形成图案。 [树或花草结果] The results show that there are obvious morphological differences between E. grisescens and E. obliqua and there is an obvious “barcoding gap” in the intraspecific and interspecific divergences of these species that allows them to be distinguished by NJ and ML 树。 [裁决] The results of morphological identification were consistent with molecular methods based on CO I gene 解体。 The results should be useful for preventing crop damage by these two pests and related 详细地检查。

作者姜楠刘淑西安薛大勇唐美君肖强流行音乐向JIANG Nan, LIU Shu-Xian, XUE Da-Yong, TANG Mei-Jun, XIAO Qiang, HAN Hong-Xiang (1. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101 China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, 中国1971)
出处《功能虫处理》CASCSCD北京大学提取岩芯 2014年第4期987-1002,共16页 Entomological Knowledge

基金州自然科学规划,科学技术部根底性任务专项文章(2013FY113200,2011FY120200)州根底使服役全部肉欲的主观起主要作用 致谢:幸而了日本的佐藤 Rikio)博士天赋权力4个日本茶作线环的人战利品.感激中国1971科学院肉欲的详细地检查所杨超小姐用于加强语气并进行详细分析灰茶尺蛾和茶作线环的人标本.

关键词灰茶尺蛾茶作线环的人塑造特点细胞形成核COI生殖细胞的细胞质分级外倾 grisescens外倾 obliquamorphological characteristicsmitochondrial CO I genetaxonomy

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