我国华东地区两种茶尺蛾的形态和分子鉴定

摘要我国华东地区两种朴素的为害茶树的益虫灰茶尺蛾Ectropin grisescens Warren,1894和茶作线环的人睑外翻 obliqua (普罗特,1915)同次多项式相像性、分类学不清,农业生产中常常污迹,它给引领和引领任务的开展风浪区了相当大的拮据。。为了正确的辨别灰茶尺蛾和茶作线环的人,两种益虫Carri的状态观看和分子剖析。对灰茶尺蛾和茶作线环的人成熟的、卵、对照剖析了将某物挖出和蛹的状态特点。;并对灰茶尺蛾和茶作线环的人的39个范本的线粒体的COI生殖细胞的细胞质举行了振幅和测序,两个物种经过的种内和种间遗传间隔为,因为Kimura-2限制因素从事制造模型,用ML和NJ方式构造体系树。灰茶尺蛾和茶作线环的人一份遗产状态学特点具有明显多样化;分子创纪录的显示,在T细胞中有任何人明显的条形码。 gap”,敷用药ML和NJ法构造的体系发育树可以将灰茶尺蛾和茶作线环的人成的区别开。因为COI生殖细胞的细胞质的分子评议出路与MORP划一。,可用于益虫防治及相互关系认为如何。。睑外翻 grisescens Warren, 1894 and 睑外翻 obliqua (普罗特,1915),two serious pests of tea in south-eastern China, are morphologically similar and cannot easily be distinguished, which makes it difficult to conduct research on these 益虫。 To accurately identify E. grisescens and E. obliqua,we examined and compared morphological characters and the mitochondrial CO I gene sequences of these two 种。 [方式] Morphological characters of adults, eggs, larvae and pupae of each species were observed, illustrated, measured and 比拟。 39 samples of the mitochondrial CO I gene of E. grisescens and E. obliqua were amplified and sequenced, and the intraspecific and interspecific divergences of the two species were 计算。 NJ and ML trees were built based on the Kimura-2-parameter 从事制造模型。 [出路] The results show that there are obvious morphological differences between E. grisescens and E. obliqua and there is an obvious “barcoding gap” in the intraspecific and interspecific divergences of these species that allows them to be distinguished by NJ and ML 树。 [收场诗] The results of morphological identification were consistent with molecular methods based on CO I gene 变形。 The results should be useful for preventing crop damage by these two pests and related 认为如何。

作者姜楠刘淑西安薛大勇唐美君肖强流行音乐向JIANG Nan, LIU Shu-Xian, XUE Da-Yong, TANG Mei-Jun, XIAO Qiang, HAN Hong-Xiang (1. Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101 China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, 奇纳)
出处《敷用药虫杂志》CASCSCD北京大学小瘤 2014年第4期987-1002,共16页 Entomological Knowledge

基金地区自然科学文章,科学技术部根底性任务专项文章(2013FY113200,2011FY120200)地区根底委员创造物特性提出特有的 致谢:由于了日本的佐藤 Rikio)博士天赋权力4个日本茶作线环的人战利品.感奇纳科学院创造物认为如何所杨超女儿从事制造并切段灰茶尺蛾和茶作线环的人标本.

关键词灰茶尺蛾茶作线环的人状态特点线粒体的COI生殖细胞的细胞质分类学睑外翻 grisescens睑外翻 obliquamorphological characteristicsmitochondrial CO I genetaxonomy

作者簡介 对应作者,xqtea@vip.163.com E-mail:hanhx@ioz.ac.cn;

 

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